Reigning champions Liverpool, who secured progress from the group stage with a matchday six win over Red Bull Salzburg, have been drawn against 2014 and 2016 finalists Atletico Madrid in what promises to be a very difficult tie for both teams.
13-time European champions Real Madrid finished second in their group and so will have to face Premier League champions Manchester City and former adversary Pep Guardiola.
Last season’s finalists, Tottenham Hotspur, are to face RB Leipzig, who are in the Champions League knockout stages for the first time.
Paris Saint-Germain coach Thomas Tuchel will face his former club Borussia Dortmund.
The eight group winners were seeded for the draw, each facing a group runner-up. Usual last 16 rules applied, with no team able to face a side that qualified from their group. Country protection is also still in operation at this stage of the competition.
2019/20 UEFA Champions League Last 16 Draw:
Borussia Dortmund vs Paris Saint-Germain (18 February & 11 March)
Real Madrid vs Manchester City (26 February & 17 March)
Atalanta vs Valencia (19 February & 10 March)
Atletico Madrid vs Liverpool (18 February & 11 March)
Chelsea vs Bayern Munich (25 February & 18 March)
Lyon vs Juventus (26 February & 17 March)
Tottenham Hotspur vs RB Leipzig (19 February & 10 March)
Napoli vs Barcelona (25 February & 18 March)
The team drawn first (unseeded) will play the first leg at home.
The full draw for the quarter-finals and semi-finals and remaining knockout bracket will then be made on 20 March. The final will take at the Ataturk Olympic Stadium in Istanbul on 30 May.