The brothers Higuain starred for Inter Miami on Saturday as David Beckham's franchise earned a historic first ever MLS win.
Elsewhere, 17-year-old Cade Cowell grabbed the headlines for San Jose Earthquakes against FC Dallas while the Sunday evening game saw Javier Hernandez fire in a stunning hat-trick against New York Red Bulls.
Miami lost their first game in a thriller against LA Galaxy on opening day and it had seemed as if they were heading for another defeat with 20 minutes remaining against Philadelphia Union. But then the Higuains came to the rescue.
Ex-Real Madrid striker Gonzalo was the marquee signing in the summer, but it was his older brother Federico who was the MVP on the day. Trailing 1-0 to a Jamiro Monteiro strike, the 36-year-old came off the bench to assist his brother before netting the winner himself just a few minutes from time.
Stoke City hall of famer Ryan Shawcross was also on the pitch.
It was the next generation who stole the show as San Jose Earthquakes beat Dallas, however. Cade Cowell doesn't turn 18 until October, but scored one and assisted another as Quakes picked up a comfortable 3-1 win.
The final match of the round saw LA Galaxy conquer New York Red Bulls in a meeting between two of MLS' best known clubs.
Javier Hernandez was the man of the moment, netting a signature poacher's hat-trick after just an hour of football to ensure that Galaxy became the only team to start the MLS season with back to back wins. Red Bulls made it uncomfortable, with Christian Casseres Jr. setting up a nervy finish, but the points eventually went to the home team.
New York City were at their best to dismantle Cincinnati, with Jesus Medina and the wonderfully-named Gudmundur Thorarinsson among the goals in a 5-0 rout. But it's Montreal Impact who top the Western Conference after two games following their 2-2 draw with Nashville.
LAFC couldn't follow up their impressive opening weekend as they were held to a draw by Seattle Sounders on home turf. Eduard Atuesta opened the scoring for the home team within two minutes, but former Liverpool left-back Brad Smith hit back for the visitors in the second half.
Elsewhere, there were wins for New England, Real Salt Lake, Atlanta United, Austin and Portland.
The second round of fixtures concludes on Sunday as LA Galaxy take on New York Red Bulls in a titanic clash between two of MLS' most historic teams.