Ajax lost 2-1 to rivals PSV Eindhoven in the KNVB Cup final on Sunday in Rotterdam.
The Amsterdam outfit surrendered a one-goal lead and conceded two early second-half strikes to hand PSV the first silverware of the Dutch season.
Ajax boss Erik ten Hag was bombarded with questions regarding his summer switch to Manchester United prior to the final and will not have been happy with the circumstances in which his side were beaten.
De Godenzonen took the lead midway through the first half when Ryan Gravenberch - who seems destined to join Bayern Munich - wriggled his way through the PSV midfield before curling into the bottom corner past the helpless Yvon Mvogo.
But Roger Schmidt's side, who were dumped out of the Europa Conference League by Leicester in midweek, responded brilliantly after the interval.
Erick Gutierrez headed home from a well-worked set piece minutes after the restart, before wanted man Cody Gakpo scored the winner with a super solo effort not too dissimilar to that of Gravenberch's moments later.
Ten Hag's team selection made headlines prior to kick-off after dropping goalkeeper Andre Onana and star striker Sebastien Haller, which will undoubtedly come into question off the back of such a momentous defeat.
Ajax remain in pole position to clinch this season's Eredivisie however, sitting four points clear of PSV with five games remaining.