The Blaugrana first rose to prominence on the continental stage with their 'Dream Team' of the early 90s before Guardiola took tiki-taka football to another level on memorable nights in Rome and at Wembley Stadium.
Here's a list of Barca's 30 top scorers in the Champions League.
30. Sonny Anderson
Brazilian striker Anderson only played two seasons in Catalonia, but spent most of his career in France where he amassed an impressive 138 goals in 221 Ligue 1 matches.
29. Crisitian Tello
Tello was a product of Barcelona's famed La Masia academy and went on to feature 86 times for his boyhood club, earning a one international cap.
The Brazilian joined from his native Santos in 1996, spending three years at the Camp Nou before joining Olympiacos in a £10.8m deal.
27. Arda Turan
After impressing under Diego Simeone at Atletico Madrid, big things were expected of Arda Turn. However the Turkish midfielder flopped in Barcelona, only managing 55 appearances in a three-year spell.
26. Dani Alves
One of the most decorated players in club football, Alves had a fabulous partnership with Lionel Messi during his time at Barcelona and made countless devastating runs down the right-wing from his full-back position.
25. Bojan Krkic
Once tipped to be Messi's heir, Bojan never quite lived up to his hype and ended up turning out for Mark Hughes at Stoke City.
24. Jose Mari Bakero
Bakero made over 500 senior appearances during his career, winning 17 major trophies and scoring over 150 goals.
Considered by many as one of the greatest players to ever play the game, Romario was part of Johan Cruyff's 'Dream Team' scoring 30 league goals in his first season in La Liga.
22. Ivan Rakitic
Rakitic was tasked with replacing the legendary Xavi in the Barcelona engine room and did so admirably. The Croatia international won one Champions League, four La Liga titles and four Copa Del Rey before moving back to Sevilla.
The Barca academy graduate mostly played as a back-up full-back during his time in the senior set-up before moving on to Ajax.
Deco had a glittering club career enjoying success with Porto, Barcelona and Chelsea - winning the Champions League on two occasions.
19. Ousmane Dembele
The 23-year-old winger joined Barcelona in a huge £117m move following Neymar's shock departure, but the France international has struggled to fill the Brazilian's boots thanks to several serious injuries.
18. Luis Figo
Not many players make the switch between the two El Clasico rivals but Luis Figo is arguably the most high profile of those to do so. He won two La Liga titles each with Barca and Real Madrid before lifting the Champions League with Los Blancos in 2001/02.
17. David Villa
Villa may not have been at Barcelona for long but the diminutive Spanish striker certainly left his mark. The clinical finisher was a crucial part of Guardiola's side that did the double in 2010/11.
16. Thierry Henry
After suffering defeat in the 2005/06 Champions League final at the hands of Barcelona, the Gunners legend joined the Spanish giants in search of European success. He didn't have to wait long to find it as the Frenchman formed a formidable front-three alongside Samuel Eto'o and Lionel Messi and won the UCL in his second season at the club.
15. Ronald Koeman
The Dutchman had a very respectable scoring record during his playing days despite featuring mainly as a sweeper.
14. Hristo Stoichkov
The 1994 Ballon d'Or winner was also part of Johan Cruyff's 'Dream Team' and won five La Liga crowns during his time at Camp Nou.
One of the greatest midfielders to ever pull on a Barcelona shirt, Xavi won all there is to win at club and international level.
12. Andres Iniesta
Xavi and Iniesta came as a pair during their prime years, and the Spanish duo were on a different wavelength to the majority of their opponents.
11. Gerard Pique
Pique was another member of Guardiola's all-conquering sides forming an impenetrable partnership with Carles Puyol and then Javier Mascherano in the heart of defence.
10. Javier Saviola
Saviola is the second player on this list to feature for both Barcelona and Real Madrid. The Argentine striker picked up his sole La Liga winner's medal during his time at the Bernabeu.
The maverick Brazilian entertainer lit up the Camp Nou during his five-year spell in Catalonia, winning the Ballon d'Or in 2005 and the Champions League in 2006.
The academy graduate was in the starting line-up in the 2010/11 Champions League final as Barcelona ripped Man Utd to shreds at Wembley Stadium.
7. Samuel Eto'o
The Cameroon legend was one of the deadliest forwards on the planet during his prime and scored in two Champions League finals for Barcelona.
6. Luis Enrique
The former Barcelona midfielder turned manager scored 73 goals in over 200 appearances for Barca during his playing days.
5. Patrick Kluivert
Kluivert spent six years at Barcelona but only managed to win one trophy during his time in the Catalan capital.
Neymar enjoyed the best days of his career at Barcelona forming one-third of the club's much-feared 'MSN' forward line. The Brazilian won the Champions League 2014/15, scoring in the final against Juventus.
Rivaldo is considered one of the greatest players of his generation and won the Ballon d'Or in 1999. He helped Barca to two La Liga crowns, forming a good partnership with Patrick Kluivert, but won his only Champions League trophy with AC Milan.
2. Luis Suarez
The prolific Uruguayan striker joined Barcelona from Liverpool in 2014 for £73m and was an instant his at Camp Nou. He won the treble in his first season and left as the club's third-highest all-time goalscorer.
1. Lionel Messi
Who else but Lionel Messi? There are simply no words to describe the genius of the little magician. His goal record is ridiculous and may never be matched in Barcelona colours.