Maradona and Messi are seen Argentina's two greatest footballers, with the former winning the World Cup with La Albiceleste in 1986 and the latter picking up five Ballon d'Or awards.
Messi has long been criticised for failing to reproduce his Barcelona form when he's on international duty and hasn't played for Argentina since they exited the 2018 World Cup.
While appearing on 'The Last Word Mexico' television programme, Maradona launched into a tirade against the Barcelona man and slammed his leadership skills.
He added: "Before speaking to the manager, he will be on the PlayStation and then on the field, he asks to be and wants to be the leader.
"He is the best in the world along with Cristiano, (but) it's useless to make a leader of a guy who goes to the bathroom 20 times before a game."
The reference was believed to be in connection with Messi’s extreme nerves before games that have seen him vomit ahead of some important matches.
Messi has claimed every possible honour with Barcelona but has been on the losing side in all four of his finals with Argentina - three in the Copa America and at the 2014 World Cup.
After another disappointing tournament in Russia this year, where Argentina failed to get past the last 16, Messi opted to take a sabbatical from international football. Maradona added that he wouldn't recall Messi to the national team.
"I wouldn't call Messi but never say never...y
Argentina defeated Iraq 4-0 on Thursday with a new-look side. However, a