The Argentine superstar was not included in squad, along with Samuel Umtiti out through injury, whilst Sergi Roberto is still suspended after his lash out at Marcelo in the Clasico.
Despite Messi's absence from the squad, Barcelona's leading goalscorer looks set for his fifth Pichichi Trophy, bringing him level with Real Madrid legend Alfredo Di Stefano and one behind the record holder Telmo Zarra.
Barcelona go into the game unbeaten in La Liga, dropping just 18 points all season. However, Levante are in good stead to try and break that record, with Granotas winning their last four home games.
Messi, currently on 34 goals, will miss only his second La Liga game of the season, the first being when his son Ciro was born, forcing him to home match against Malaga.